Factors Limiting Sexual Reproduction in Platanus Wrightii in Southeastern Arizona

Arizona sycamore (Platanus wrightii: Platanaceae) is a riparian tree of the southwestern United States and adjacent Mexico. It is failing to reproduce in certain canyons where mature, seed-producing trees of this species occur. Three hypotheses were tested to explain this reproductive failure: I) the presence of domestic cattle prevents reproduction, 2) seeds produced in certain canyons are inviable, and 3) annual flash floods destroy seedlings and young saplings but not the large, mature trees. Canyons, either grazed or ungrazed by domestic animals, were surveyed for the presence of seedlings and young trees. In the laboratory, seeds were tested for viability, germinability in petri dishes, and emergence of seedlings from soil. Canyons which possessed seedlings and young saplings were censused before and after flooding. From these efforts, we conclude that reproductive failure of Arizona sycamore in certain canyons cannot be explained either by activities of domestic animals or by a lack of viable, germinable seeds. Flash flooding events in some canyons washed out the seedlings and saplings present, but left viable larger trees. We also found that a permanent, high water table was essential to propagule survival

Wide Field Aperture Synthesis Radio Astronomy

This thesis is focussed on the Molonglo Observatory Synthesis Telescope (MOST), reporting on two primary areas of investigation. Firstly, it describes the recent upgrade of the MOST to perform an imaging survey of the southern sky. Secondly, it presents a MOST survey of the Vela supernova remnant and follow-up multiwavelength studies. The MOST Wide Field upgrade is the most significant instrumental upgrade of the telescope since observations began in 1981. It has made possible the nightly observation of fields with area ~5 square degrees, while retaining the operating frequency of 843 MHz and the pre-existing sensitivity to point sources and extended structure. The MOST will now be used to make a sensitive (rms approximately 1 mJy/beam) imaging survey of the sky south of declination -30°. This survey consists of two components: an extragalactic survey, which will begin in the south polar region, and a Galactic survey of latitudes |b| < 10°. These are expected to take about ten years. The upgrade has necessitated the installation of 352 new preamplifiers and phasing circuits which are controlled by 88 distributed microcontrollers, networked using optic fibre. The thesis documents the upgrade and describes the new systems, including associated testing, installation and commissioning. The thesis continues by presenting a new high-resolution radio continuum survey of the Vela supernova remnant (SNR), made with the MOST before the completion of the Wide Field upgrade. This remnant is the closest and one of the brightest SNRs. The contrast between the structures in the central pulsar-powered nebula and the synchrotron radiation shell allows the remnant to be identified morphologically as a member of the composite class. The data are the first of a composite remnant at spatial scales comparable with those available for the Cygnus Loop and the Crab Nebula, and make possible a comparison of radio, optical and soft X-ray emission from the resolved shell filaments. The survey covers an area of 50 square degrees at a resolution of 43" x 60", while imaging structures on scales up to 30'. It has been used for comparison with Wide Field observations to evaluate the performance of the upgraded MOST. The central plerion of the Vela SNR (Vela X) contains a network of complex filamentary structures. The validity of the imaging of these filaments has been confirmed with Very Large Array (VLA) observations at 1.4 GHz. Unlike the situation in the Crab Nebula, the filaments are not well correlated with H-alpha emission. Within a few parsec of the Vela pulsar the emission is much more complex than previously seen: both very sharp edges and more diffuse emission are present. It has been postulated that one of the brightest filaments in Vela X is associated with the X-ray feature (called a `jet') which appears to be emanating from the region of the pulsar. However, an analysis of the MOST and VLA data shows that this radio filament has a flat spectral index similar to another more distant filament within the plerion, indicating that it is probably unrelated to the X-ray feature

An improved vertical correction method for the inter-comparison and inter-validation of integrated water vapour measurements

Integrated water vapour (IWV) measurements from similar or different techniques are often inter-compared for calibration and validation purposes. Results are usually assessed in terms of bias (difference of the means), standard deviation of the differences, and linear fit slope and offset (intercept) estimates. When the instruments are located at different elevations, a correction must be applied to account for the vertical displacement between the sites. Empirical formulations are traditionally used for this correction. In this paper we show that the widely used correction model based on a standard, exponential, profile for water vapour cannot properly correct the bias, slope, and offset parameters simultaneously. Correcting the bias with this model degrades the slope and offset estimates and vice versa. This paper proposes an improved correction method that overcomes these limitations. It implements a multiple linear regression method where the slope and offset parameters are provided from a radiosonde climatology. It is able to predict monthly mean IWVs with a bias smaller than 0.1 kg m−2 and a root-mean-square error smaller than 0.5 kg m−2 for height differences up to 500 m. The method is applied to the inter-comparison of GPS IWV data in a tropical mountainous area and to the inter-validation of GPS and satellite microwave radiometer data. This paper also emphasizes the need for using a slope and offset regression method that accounts for errors in both variables and for correctly specifying these errors.</p

ECG Beat Representation and Delineation by Means of Variable Projection

Objective: The electrocardiogram (ECG) follows a characteristic shape, which has led to the development of several mathematical models for extracting clinically important information. Our main objective is to resolve limitations of previous approaches, that means to simultaneously cope with various noise sources, perform exact beat segmentation, and to retain diagnostically important morphological information. Methods: We therefore propose a model that is based on Hermite and sigmoid functions combined with piecewise polynomial interpolation for exact segmentation and low-dimensional representation of individual ECG beat segments. Hermite and sigmoidal functions enable reliable extraction of important ECG waveform information while the piecewise polynomial interpolation captures noisy signal features like the baseline wander (BLW). For that we use variable projection, which allows the separation of linear and nonlinear morphological variations of the according ECG waveforms. The resulting ECG model simultaneously performs BLW cancellation, beat segmentation, and low-dimensional waveform representation. Results: We demonstrate its BLW denoising and segmentation performance in two experiments, using synthetic and real data. Compared to state-of-the-art algorithms, the experiments showed less diagnostic distortion in case of denoising and a more robust delineation for the P and T wave. Conclusion: This work suggests a novel concept for ECG beat representation, easily adaptable to other biomedical signals with similar shape characteristics, such as blood pressure and evoked potentials. Significance: Our method is able to capture linear and nonlinear wave shape changes. Therefore, it provides a novel methodology to understand the origin of morphological variations caused, for instance, by respiration, medication, and abnormalities

Variación morfológica en Bolitoglossa vallecula (Amphibia: Caudata: Plethodontidae) en la Cordillera Central de Colombia

ABSTRACT: Body size and head shape variation was quanti ed in four populations of Bolitoglossa vallecula in the Cordillera Central of Colombia. two of these four populations occurred in sympatry with B. ramosi, an ecologically similar salamander species that could potentially compete with B. vallecula for food resources. there was no evidence of sexual size or head shape dimorphism in any population, so data for males and females were pooled. the populations differed signi cantly in mean body size, but this variation was not associated with elevation. Six head shape characteristics associated with head morphology were quantified and analyzed using the ratio of each variable over the geometric median, given that this is the most preferred method in the literature for describing conformation while controlling for the effects of size. One of the populations sympatric with B. ramosi (Bello) exhibited signficant differences in conformation of head morphology when compared to the allopatric populations, while the other sympatric population did not. While the evidence for character displacement due to ecological competition was equivocal, the analyses highlight areas where further research should be directed.RESUMEN: Cuantificamos la variación en el tamaño y forma del cuerpo y cabeza de Bolitoglossa vallecula en cuatro poblaciones de la Cordillera Central de Colombia. Dos de estas cuatro poblaciones ocurren en simpatría con B. ramosi una especie de salamandra ecológicamente similar que podría potencialmente competir con B. vallecula por recursos alimenticios. En ninguna de las poblaciones encontramos evidencias de dimorfismo sexual en el tamaño o forma de la cabeza, por lo tanto los datos de hembras y machos fueron agrupados. Las poblaciones di rieron significativamente en el tamaño promedio del cuerpo, pero esta variación no estuvo asociada con la elevación. Cuantificamos seis características de la forma de la cabeza asociadas con la morfología de la misma y las analizamos utilizando las proporciones de cada variable sobre la media geométrica, debido a que esta metodología es la más utilizada en la literatura para describir conformación mientras se controla por el efecto del tamaño. Una de las poblaciones simpátricas con B. ramosi (Bello) presentó diferencias significativas en la conformación de la morfología de la cabeza cuando se comparó con las poblaciones alopátricas, mientras que la otra población simpátrica no. Aunque en este trabajo la evidencia de desplazamiento de caracteres en respuesta a la competencia ecológica no es concluyente, este análisis destaca áreas de investigación que vale la pena adelantar en el futuro

Mercury Levels in Eggs, Embryos, and Neonates of Trachemys callirostris (Testudines, Emydidae)

RESUMEN: Cuantificamos la concentración total de mercurio en cáscaras, yemas y embriones de 16 nidos de hicotea (Trachemys callirostris). Los nidos fueron colectados en diferentes estadios de desarrollo embrionario. No hubo una correlación entre el tiempo estimado desde el desove y los niveles de mercurio en los huevos, sugiriendo que el metal no fue absorbido del substrato, sino que probablemente éste fue transferido a los huevos durante el proceso de foliculogénesis en las hembras reproductivas, las cuales bioacumularon el mercurio de fuentes ambientales. La concentración promedio de mercurio fue mayor en los embriones que en las cáscaras o yemas, indicando que los embriones también bioacumulan el metal presente en otros tejidos del huevo. La variación de la concentración de mercurio dentro de una misma nidada fue relativamente alta. Las concentraciones de mercurio en las yemas no estuvieron asociadas con ninguna de las medidas de fitness que fueron evaluadas (éxito de eclosión, tamaño inicial de los neonatos y tasas de crecimiento de los juveniles en el primer mes). Después de cinco meses de mantenimiento en cautiverio, en un ambiente libre de mercurio, 86 % de los juveniles había eliminado completamente este metal de sus tejidos.ABSTRACT: We quantified total mercury concentrations in eggshells, egg yolks, and embryos from 16 nests of the Colombian Slider (Trachemys callirostris). Nests were collected in different stages of development, but estimated time of incubation in natural substrates was not correlated with mercury levels in the eggs, suggesting that mercury was not absorbed from the substrate, but more likely passed on to the embryos during folliculogenesis by the reproductive females who had bioaccumulated the mercury from environmental sources. Mean mercury concentrations were higher in embryos than in eggshells or egg yolks, indicating that embryos also bioaccumulate mercury present in other egg tissues. Intra-clutch variation in egg yolk mercury concentrations was relatively high. Egg yolk mercury concentrations were not associated with any of the fitness proxies we quantified for the nests (hatching success rates, initial neonate sizes and first-month juvenile growth rates). After five months of captive rearing in a mercury-free laboratory environment, 86 % of the juveniles had eliminated the mercury from their tissues

The Allen Telescope Array: The First Widefield, Panchromatic, Snapshot Radio Camera for Radio Astronomy and SETI

The first 42 elements of the Allen Telescope Array (ATA-42) are beginning to deliver data at the Hat Creek Radio Observatory in Northern California. Scientists and engineers are actively exploiting all of the flexibility designed into this innovative instrument for simultaneously conducting surveys of the astrophysical sky and conducting searches for distant technological civilizations. This paper summarizes the design elements of the ATA, the cost savings made possible by the use of COTS components, and the cost/performance trades that eventually enabled this first snapshot radio camera. The fundamental scientific program of this new telescope is varied and exciting; some of the first astronomical results will be discussed.Comment: Special Issue of Proceedings of the IEEE: "Advances in Radio Telescopes", Baars,J. Thompson,R., D'Addario, L., eds, 2009, in pres

Ageing-associated DNA methylation dynamics are a molecular readout of lifespan variation among mammalian species.

BACKGROUND: Mammalian species exhibit a wide range of lifespans. To date, a robust and dynamic molecular readout of these lifespan differences has not yet been identified. Recent studies have established the existence of ageing-associated differentially methylated positions (aDMPs) in human and mouse. These are CpG sites at which DNA methylation dynamics show significant correlations with age. We hypothesise that aDMPs are pan-mammalian and are a dynamic molecular readout of lifespan variation among different mammalian species. RESULTS: A large-scale integrated analysis of aDMPs in six different mammals reveals a strong negative relationship between rate of change of methylation levels at aDMPs and lifespan. This relationship also holds when comparing two different dog breeds with known differences in lifespans. In an ageing cohort of aneuploid mice carrying a complete copy of human chromosome 21, aDMPs accumulate far more rapidly than is seen in human tissues, revealing that DNA methylation at aDMP sites is largely shaped by the nuclear trans-environment and represents a robust molecular readout of the ageing cellular milieu. CONCLUSIONS: Overall, we define the first dynamic molecular readout of lifespan differences among mammalian species and propose that aDMPs will be an invaluable molecular tool for future evolutionary and mechanistic studies aimed at understanding the biological factors that determine lifespan in mammals